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  • Development and validation of a hydrophilic interaction chromatography-mass spectrometry assay for taurine and methionine in matrices rich in carbohydrates.

Development and validation of a hydrophilic interaction chromatography-mass spectrometry assay for taurine and methionine in matrices rich in carbohydrates.

Journal of chromatography. A (2005-07-26)
Marine de Person, Aurélie Hazotte, Claire Elfakir, Michel Lafosse
ABSTRACT

A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in carbohydrates such as energy drinks. No initial clean-up procedure and no sample derivatisation are required. Satisfactory analysis was obtained on an Astec apHera NH2 (150 mm x 4.6 mm; 5 microm) column with methanol-water (60/40) as mobile phase. The method was validated in terms of specificity, detection limits, linearity, accuracy, precision and stability, using threonine as internal standard. The potential effects of matrix and endogenous amino acid content were also examined. The limits of detection in the beverage varied from 20 microg L(-1) for taurine to 50 micro L(-1) for methionine.

MATERIALS
Product Number
Brand
Product Description

Supelco
apHera NH2 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
apHera NH2 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
apHera NH2 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2 mm
Supelco
apHera NH2 HPLC Guard Column, 5 μm particle size, L × I.D. 1 cm × 2 mm
Supelco
apHera NH2 HPLC Guard Column, 5 μm particle size, L × I.D. 1 cm × 4.6 mm