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  • Callus induction and in vitro plant regeneration of rice (Oryza sativa L.) under various conditions.

Callus induction and in vitro plant regeneration of rice (Oryza sativa L.) under various conditions.

Pakistan journal of biological sciences : PJBS (2008-09-27)
Muhammad Tariq, Gowher Ali, Fazal Hadi, Shakeel Ahmad, Nasir Ali, Aftab Ali Shah
ABSTRACT

The objective of the present study was to develop an effective protocol for optimum callus induction and complete plant regeneration for four varieties of rice (Oryza sativa L.) i.e., Super Basmati, Basmati-370, Basmati-371 and Fakhre Malakand. Calli were induced from mature seed scutelum. The Murashige and Skoog (MS) and Chu's N6 media containing hormone 2, 4-D (2, 4-Dichlorophenoxy acetic acid) in different concentrations were used for callus induction. Fakhre Malakand produced maximum calli on N6 media containing 3 mg L(-1) 2,4-D. while other three varieties showed maximum callus induction on N6 media containing 2.5 mg L(-1) 2,4-D. N6 media was found better than MS media for callus induction. For complete plant regeneration the calli of two varieties i.e., Basmati-370 and Basmati-371 were plated on N6 media containing different concentrations of NAA (1-Naphthalene acetic acid) and BAP (6-benzyl aminopurine). The maximum regeneration frequency (%) was observed on N6 media containing NAA 1 mg L(-1) and BAP 2.5 mg L(-1). It took 27-30 days for the callus to regenerate into a complete plant. Basmati-370 produced 4-7 plantlets per callus whereas Basmati-371 produced 4-8 plantlets per callus with regeneration frequencies of 61 and 69%, respectively.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
6-Benzylaminopurine, suitable for plant cell culture
Sigma-Aldrich
6-Benzylaminopurine solution, 1 mg/mL, suitable for plant cell culture
Sigma-Aldrich
1-Naphthaleneacetic acid, suitable for plant cell culture, BioReagent, ≥95%, crystalline
Sigma-Aldrich
1-Naphthylacetic acid, 1 mg/mL, BioReagent, suitable for plant cell culture
Sigma-Aldrich
Chu (N6) Basal Salt Mixture, powder, suitable for plant cell culture