• Alterations in gut microbiota associated with a cafeteria diet and the physiological consequences in the host.

Alterations in gut microbiota associated with a cafeteria diet and the physiological consequences in the host.

International journal of obesity (2005) (2017-11-24)
J M Del Bas, M Guirro, N Boqué, A Cereto, R Ras, A Crescenti, A Caimari, N Canela, L Arola

Gut microbiota have been described as key factors in the pathophysiology of obesity and different components of metabolic syndrome (MetS). The cafeteria diet (CAF)-fed rat is a preclinical model that reproduces most of the alterations found in human MetS by simulating a palatable human unbalanced diet. Our objective was to assess the effects of CAF on gut microbiota and their associations with different components of MetS in Wistar rats. Animals were fed a standard diet or CAF for 12 weeks. A partial least square-based methodology was used to reveal associations between gut microbiota, characterized by 16S ribosomal DNA gene sequencing, and biochemical, nutritional and physiological parameters. CAF feeding resulted in obesity, dyslipidemia, insulin resistance and hepatic steatosis. These changes were accompanied by a significant decrease in gut bacterial diversity, decreased Firmicutes and an increase in Actinobacteria and Proteobacteria abundances, which were concomitant with increased endotoxemia. Associations of different genera with the intake of lipids and carbohydrates were opposed from those associated with the intake of fiber. Changes in gut microbiota were also associated with the different physiological effects of CAF, mainly increased adiposity and altered levels of plasma leptin and glycerol, consistent with altered adipose tissue metabolism. Also hepatic lipid accretion was associated with changes in microbiota, highlighting the relevance of gut microbiota homeostasis in the adipose-liver axis. Overall, our results suggest that CAF feeding has a profound impact on the gut microbiome and, in turn, that these changes may be associated with important features of MetS.

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DL-β-Hydroxylauric acid, ≥99% (GC)
3-Hydroxytetradecanoic acid-2,2,3,4,4-d5, 98 atom % D
(±)-3-Hydroxydecanoic acid, ≥98%
DL-β-Hydroxypalmitic acid, ≥98%
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