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  • Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery.

Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery.

Bioconjugate chemistry (2007-09-13)
Xue-Qing Zhang, Janjira Intra, Aliasger K Salem
ABSTRACT

We report on the preparation and characterization of poly(D, L-lactide-co-glycolide) (PLGA) microparticles with surface-conjugated polyamidoamine (PAMAM) dendrimers of varying generations. The buffering capacity and zeta-potential of the PLGA PAMAM microparticles increased with increasing generation level of the PAMAM dendrimer conjugated. Conjugation of the PAMAM dendrimer to the surface of the PLGA microparticle removed generation-dependent cytotoxicity in HEK293 and COS7 cell lines. PLGA PAMAM pDNA microparticles displayed similar cytotoxicity profiles to unmodified PLGA pDNA microparticles in COS7 cells. A generation three PAMAM dendrimer conjugated to PLGA microparticles significantly increased transfection efficiencies in comparison to unmodified PLGA microparticles.

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Sigma-Aldrich
Heparin−biotin sodium salt, ≥97%