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  • Self-assembled terplexes for targeted gene delivery with improved transfection.

Self-assembled terplexes for targeted gene delivery with improved transfection.

Bioconjugate chemistry (2010-10-12)
Qiao Zhang, Si Chen, Ren-Xi Zhuo, Xian-Zheng Zhang, Si-Xue Cheng
ABSTRACT

To improve transfection efficiency and to incorporate target ligands to the gene delivery systems, heparin and heparin-biotin were introduced to complexes of polyamidoamine dendrimer and DNA (PAMAM/DNA) via electrostatic interactions to form self-assembled PAMAM/DNA/heparin and PAMAM/DNA/heparin-biotin terplexes, respectively. The self-assembled terplexes were characterized by agarose gel electrophoresis and particle size analysis. The MTT assay indicated that, after incorporation of heparin and heparin-biotin, the terplexes exhibited decreased cytotoxicity. In addition, as compared with PAMAM/DNA and PAMAM/DNA/heparin complexes, the PAMAM/DNA/heparin-biotin complexes exhibited much higher cellular uptake into HeLa cells due to the specific interactions between biotin and biotin receptors on HeLa cells, which led to the enhanced transfection activity. The PAMAM/DNA/heparin-biotin complexes would be a promising targeting gene delivery system.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Heparin−biotin sodium salt, ≥97%