MilliporeSigma
  • An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells.

An Integrative Analysis of microRNA and mRNA Profiling in CML Stem Cells.

Methods in molecular biology (Clifton, N.J.) (2016-09-02)
Farah J Nassar, Rabab El Eit, Rihab Nasr
ABSTRACT

Integrative analysis of microRNA (miRNA) and messenger RNA (mRNA) in Chronic Myeloid leukemia (CML) stem cells is an important technique to study the involvement of miRNA and their targets in CML stem cells self-renewal, maintenance, and therapeutic resistance. Here, we describe a simplified integrative analysis using Ingenuity Pathway Analysis software after performing proper RNA extraction, miRNA and mRNA microarray and data analysis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
RNaseZAP, Cleaning agent for removing RNase