MilliporeSigma
  • Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10.

Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10.

Cancer research (1990-09-25)
H D Soule, T M Maloney, S R Wolman, W D Peterson, R Brenz, C M McGrath, J Russo, R J Pauley, R F Jones, S C Brooks
ABSTRACT

Two sublines of a breast epithelial cell culture, MCF-10, derived from human fibrocystic mammary tissue exhibit immortality after extended cultivation in low calcium concentrations (0.03-0.06 mM) and floating transfers in low calcium (MCF-10F), or by trypsin-Versene passages in the customary (normal) calcium levels, 1.05 mM (MCF-10A). Both sublines have been maintained as separate entities after 2.3 years (849 days) in vitro and at present have been in culture for longer than 4 years. MCF-10 has the characteristics of normal breast epithelium by the following criteria: (a) lack of tumorigenicity in nude mice; (b) three-dimensional growth in collagen; (c) growth in culture that is controlled by hormones and growth factors; (d) lack of anchorage-independent growth; and (e) dome formation in confluent cultures. Cytogenetic analysis prior to immortalization showed normal diploid cells; although later passages showed minimal rearrangement and near-diploidy, the immortal cells were not karyotypically normal. The emergence of an immortal culture in normal calcium media was not an inherent characteristic of the original tissue from which MCF-10 was derived since reactivated cryo-preserved cells from cultures grown for 0.3 and 1.2 years in low calcium were incapable of sustained growth in normal calcium.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
UFH-001 Human Breast Cancer Cell Line, The UFH-001 triple negative CAIX+ human breast cancer cell line is suitable as a model for studying the mechanisms of triple negative breast cancer progression, metastasis and migration.