Skip to Content
MilliporeSigma
  • Hedgehog Signaling Regulates Neurogenesis in the Larval and Adult Zebrafish Hypothalamus.

Hedgehog Signaling Regulates Neurogenesis in the Larval and Adult Zebrafish Hypothalamus.

eNeuro (2020-10-28)
Ira Male, A Tuba Ozacar, Rita R Fagan, Matthew D Loring, Meng-Chieh Shen, Veronica A Pace, Christine A Devine, Grace E Lawson, Alyssa Lutservitz, Rolf O Karlstrom
ABSTRACT

Neurogenesis is now known to play a role in adult hypothalamic function, yet the cell-cell mechanisms regulating this neurogenesis remain poorly understood. Here, we show that Hedgehog (Hh)/Gli signaling positively regulates hypothalamic neurogenesis in both larval and adult zebrafish and is necessary and sufficient for normal hypothalamic proliferation rates. Hh-responsive radial glia represent a relatively highly proliferative precursor population that gives rise to dopaminergic, serotonergic, and GABAergic neurons. In situ and transgenic reporter analyses revealed substantial heterogeneity in cell-cell signaling within the hypothalamic niche, with slow cycling Nestin-expressing cells residing among distinct and overlapping populations of Sonic Hh (Shh)-expressing, Hh-responsive, Notch-responsive, and Wnt-responsive radial glia. This work shows for the first time that Hh/Gli signaling is a key component of the complex cell-cell signaling environment that regulates hypothalamic neurogenesis throughout life.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse, clone PC 10, ascites fluid
Roche
DIG DNA Labeling and Detection Kit
Sigma-Aldrich
Anti-Sox2 Antibody, Chemicon®, from rabbit