Skip to Content
MilliporeSigma
  • Persistence of a Yeast-Based (Hanseniaspora uvarum) Attract-and-Kill Formulation against Drosophila suzukii on Grape Leaves.

Persistence of a Yeast-Based (Hanseniaspora uvarum) Attract-and-Kill Formulation against Drosophila suzukii on Grape Leaves.

Insects (2020-11-22)
Flavia Bianchi, Urban Spitaler, Irene Castellan, Carlo S Cossu, Timothy Brigadoi, Claire Duménil, Sergio Angeli, Peter Robatscher, Rudi F Vogel, Silvia Schmidt, Daniela Eisenstecken
ABSTRACT

The production of phagostimulant and attractive volatile organic compounds (VOCs) by yeasts can be exploited to improve the efficacy of attract-and-kill formulations against the spotted wing drosophila (SWD). This study evaluated the persistence over one week of a yeast-based formulation under greenhouse conditions. Potted grape plants were treated with: (i) potato dextrose broth (PDB), (ii) PDB containing spinosad (PDB + S), and (iii) H. uvarum fermentation broth grown on PDB containing spinosad (H. u. + S). Laboratory trials were performed to determine the survival and the oviposition rate of SWD after exposure to treated leaves. Ion-exchange chromatography was performed to measure carbohydrates, sugar alcohols, and organic acids on leaf surfaces, while amino acids were assessed through liquid chromatography-mass-spectrometry. Additionally, the VOCs released by plants treated with H.uvarum were collected via closed-loop-stripping analysis and compared to those emitted by untreated leaves. A higher mortality was observed for adult SWDs in contact with H. uvarum containing spinosad compared to PDB containing spinosad. Generally, a decrease in the amounts of non-volatile compounds was observed over time, though numerous nutrients were still present one week after treatment. The application of the yeast-based formulation induced the emission of VOCs by the treated leaves. The concentration of 2-phenylethanol, one of the main VOCs emitted by yeasts, decreased over time. These findings describe the presence of potential phagostimulants and compounds attractive to SWD in a yeast-based attract-and-kill formulation and demonstrate the efficacy of the formulation over one week.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DL-Phenylalanine-3,3-d2, 98 atom % D
Sigma-Aldrich
L-Alanine-2,3,3,3-d4, 98 atom % D
Sigma-Aldrich
L-Glutamic acid-2,3,3,4,4-d5, 97 atom % D, 98% (CP)
Sigma-Aldrich
L-Lysine-4,4,5,5-d4 hydrochloride, 98 atom % D, 98% (CP)