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  • Transcriptional Dynamics of Hair-Bundle Morphogenesis Revealed with CellTrails.

Transcriptional Dynamics of Hair-Bundle Morphogenesis Revealed with CellTrails.

Cell reports (2018-06-07)
Daniel C Ellwanger, Mirko Scheibinger, Rachel A Dumont, Peter G Barr-Gillespie, Stefan Heller
ABSTRACT

Protruding from the apical surface of inner ear sensory cells, hair bundles carry out mechanotransduction. Bundle growth involves sequential and overlapping cellular processes, which are concealed within gene expression profiles of individual cells. To dissect such processes, we developed CellTrails, a tool for uncovering, analyzing, and visualizing single-cell gene-expression dynamics. Utilizing quantitative gene-expression data for key bundle proteins from single cells of the developing chick utricle, we reconstructed de novo a bifurcating trajectory that spanned from progenitor cells to mature striolar and extrastriolar hair cells. Extraction and alignment of developmental trails and association of pseudotime with bundle length measurements linked expression dynamics of individual genes with bundle growth stages. Differential trail analysis revealed high-resolution dynamics of transcripts that control striolar and extrastriolar bundle development, including those that encode proteins that regulate [Ca2+]i or mediate crosslinking and lengthening of actin filaments.

MATERIALS
Product Number
Brand
Product Description

Roche
SP6/T7 Transcription Kit, sufficient for 2 x 20 assays (stadard transcription), kit of 1 (12 components), suitable for DNA sequencing, suitable for hybridization