Skip to Content
MilliporeSigma
  • A standardised semi-dynamic in vitro digestion method suitable for food - an international consensus.

A standardised semi-dynamic in vitro digestion method suitable for food - an international consensus.

Food & function (2020-02-11)
Ana-Isabel Mulet-Cabero, Lotti Egger, Reto Portmann, Olivia Ménard, Sébastien Marze, Mans Minekus, Steven Le Feunteun, Anwesha Sarkar, Myriam M-L Grundy, Frédéric Carrière, Matt Golding, Didier Dupont, Isidra Recio, André Brodkorb, Alan Mackie
ABSTRACT

The link between food and human health is increasingly a topic of interest. One avenue of study has been to assess food disintegration and interactions within the gastrointestinal tract. In vitro digestion models have been widely used to overcome the constrictions associated with in vivo methodology. The COST Action INFOGEST developed an international, harmonised protocol for static simulation of digestion in the upper gastrointestinal tract of adults. This protocol is widely used; however, it is restricted to providing end-point assessment without considering the possible structural changes. On the other hand, there are dynamic models that provide more physiologically relevant data but are expensive and difficult to access. There is a gap between these models. The method outlined in this article provides an intermediate model; it builds upon the harmonised static model and now includes crucial kinetic aspects associated with the gastric phase of digestion, including gradual acidification, fluid and enzyme secretion and emptying. This paper provides guidance and standardised recommendations of a physiologically relevant semi-dynamic in vitro simulation of upper gastrointestinal tract digestion, with particular focus on the gastric phase. Adaptations of this model have already been used to provide kinetic data on nutrient digestion and structural changes during the gastric phase that impact on nutrient absorption. Moreover, it provides a simple tool that can be used in a wide range of laboratories.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Colipase from porcine pancreas, essentially salt-free, lyophilized powder
Pricing and availability is not currently available.
Sigma-Aldrich
Lipase from porcine pancreas, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Pricing and availability is not currently available.
Sigma-Aldrich
α-Amylase from porcine pancreas, Type VI-B, ≥5 units/mg solid
Pricing and availability is not currently available.
Sigma-Aldrich
Pepsin from porcine gastric mucosa, lyophilized powder, ≥2,500 units/mg protein (E1%/280)
Pricing and availability is not currently available.
Sigma-Aldrich
Methyltrioctylammonium hydrogen sulfate, ≥95.0% (T)
Pricing and availability is not currently available.
Sigma-Aldrich
α-Amylase from human saliva, Type XIII-A, lyophilized powder, 300-1,500 units/mg protein
Pricing and availability is not currently available.
Sigma-Aldrich
Trypsin from porcine pancreas, Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein
Pricing and availability is not currently available.
Millipore
Bile bovine, dried, unfractionated
Pricing and availability is not currently available.
Sigma-Aldrich
α-Chymotrypsin from bovine pancreas, Type I-S, essentially salt-free, lyophilized powder
Pricing and availability is not currently available.
Sigma-Aldrich
Pancreatin from porcine pancreas, 8 × USP specifications
Pricing and availability is not currently available.
Sigma-Aldrich
Orlistat, ≥98%, solid
Pricing and availability is not currently available.