Fe3+ and intracellular pH determination based on orange fluorescence carbon dots co-doped with boron, nitrogen and sulfur.

The fluorescent boron, nitrogen and sulfur co-doped carbon dots (BNSCDs) were prepared by simple hydrothermal reaction of 4-carboxyphenylboronic acid and 2,5-diaminobenzenesulfonic acid at 200 °C for 8 h. The fluorescence of the BNSCDs could be quenched by Fe3+ based on the electron transfer between Fe3+ and BNSCDs, so a label-free, good selectivity and high sensitivity method for Fe3+determination was established with linear range and LOD of 1.5-692 μmol/L and 87 nmol/L, respectively. And then the fluorescent probe was employed for detection of Fe3+ in tap water, coal gangue, fly ash and food samples successfully. Moreover, the as-prepared BNSCDs could serve as a novel pH fluorescent probe in the range of pH 1.60-7.00, which could be attributed to the proton transfer of carboxyl groups on the surface of BNSCDs. More importantly, the pH fluorescent probe possesses fast, real-time and low toxicity, applying for intracellular pH fluorescence imaging in HIC, HIEC, LO2 and SMMC7721 cells. In view of its simplicity, timely response and outstanding compatibility, the as-fabricated BNSCDs show the potential applications in water quality and solid waste monitoring, food detection, real-time measuring of intracellular pH change in vitro.