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  • High diversity of extended-spectrum beta-lactamases in Escherichia coli isolates from Italian broiler flocks.

High diversity of extended-spectrum beta-lactamases in Escherichia coli isolates from Italian broiler flocks.

Antimicrobial agents and chemotherapy (2010-01-27)
Valeria Bortolaia, Luca Guardabassi, Marcello Trevisani, Magne Bisgaard, Luciano Venturi, Anders Miki Bojesen
ABSTRACT

We characterized 67 Escherichia coli isolates with reduced susceptibility to cefotaxime or ceftiofur obtained from healthy broilers housed in five Italian farms. The bla(CTX-M-1), bla(CTX-M-32) and bla(SHV-12) beta-lactamase genes were identified on IncI1, IncN, or IncFIB plasmids. Considerable genetic diversity was detected among the extended-spectrum beta-lactamase (ESBL)-producing isolates, and we identified indistinguishable strains in unrelated farms and indistinguishable plasmids in genetically unrelated strains. The detection of highly mobile plasmids suggests a potential animal reservoir for beta-lactamase genes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tetracycline, 98.0-102.0% (HPLC)
Sigma-Aldrich
Nalidixic acid, ≥98%
Sigma-Aldrich
Ciprofloxacin, ≥98% (HPLC)