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  • Self-organized patterning of cell morphology via mechanosensitive feedback.

Self-organized patterning of cell morphology via mechanosensitive feedback.

eLife (2021-03-27)
Natalie A Dye, Marko Popović, K Venkatesan Iyer, Jana F Fuhrmann, Romina Piscitello-Gómez, Suzanne Eaton, Frank Jülicher
ABSTRACT

Tissue organization is often characterized by specific patterns of cell morphology. How such patterns emerge in developing tissues is a fundamental open question. Here, we investigate the emergence of tissue-scale patterns of cell shape and mechanical tissue stress in the Drosophila wing imaginal disc during larval development. Using quantitative analysis of the cellular dynamics, we reveal a pattern of radially oriented cell rearrangements that is coupled to the buildup of tangential cell elongation. Developing a laser ablation method, we map tissue stresses and extract key parameters of tissue mechanics. We present a continuum theory showing that this pattern of cell morphology and tissue stress can arise via self-organization of a mechanical feedback that couples cell polarity to active cell rearrangements. The predictions of this model are supported by knockdown of MyoVI, a component of mechanosensitive feedback. Our work reveals a mechanism for the emergence of cellular patterns in morphogenesis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Penicillin-Streptomycin, Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
20-Hydroxyecdysone, ≥93% (HPLC), powder
Sigma-Aldrich
Grace′s Insect Medium, With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture