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  • Frizzled 4 regulates ventral blood vessel remodeling in the zebrafish retina.

Frizzled 4 regulates ventral blood vessel remodeling in the zebrafish retina.

Developmental dynamics : an official publication of the American Association of Anatomists (2019-10-01)
Lucia Caceres, Sergey V Prykhozhij, Elizabeth Cairns, Harald Gjerde, Nicole M Duff, Keon Collett, Mike Ngo, Gheyath K Nasrallah, Christopher R McMaster, Matthew Litvak, Johane M Robitaille, Jason N Berman
ABSTRACT

Familial exudative vitreoretinopathy (FEVR) is a rare congenital disorder characterized by a lack of blood vessel growth to the periphery of the retina with secondary fibrovascular proliferation at the vascular-avascular junction. These structurally abnormal vessels cause leakage and hemorrhage, while the fibroproliferative scarring results in retinal dragging, detachment and blindness. Mutations in the FZD4 gene represent one of the most common causes of FEVR. A loss of function mutation resulting from a 10-nucleotide insertion into exon 1 of the zebrafish fzd4 gene was generated using transcription activator-like effector nucleases (TALENs). Structural and functional integrity of the retinal vasculature was examined by fluorescent microscopy and optokinetic responses. Zebrafish retinal vasculature is asymmetrically distributed along the dorsoventral axis, with active vascular remodeling on the ventral surface of the retina throughout development. fzd4 mutants exhibit disorganized ventral retinal vasculature with discernable tubular fusion by week 8 of development. Furthermore, fzd4 mutants have impaired optokinetic responses requiring increased illumination. We have generated a visually impaired zebrafish FEVR model exhibiting abnormal retinal vasculature. These fish provide a tractable system for studying vascular biology in retinovascular disorders, and demonstrate the feasibility of using zebrafish for evaluating future FEVR genes identified in humans.

MATERIALS
Product Number
Brand
Product Description

Roche
DIG RNA Labeling Mix, sufficient for 20 reactions, solution
Roche
Fluorescein RNA Labeling Mix, solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization