Skip to Content
MilliporeSigma
  • A low-protein diet induces body weight loss and browning of subcutaneous white adipose tissue through enhanced expression of hepatic fibroblast growth factor 21 (FGF21).

A low-protein diet induces body weight loss and browning of subcutaneous white adipose tissue through enhanced expression of hepatic fibroblast growth factor 21 (FGF21).

Molecular nutrition & food research (2017-01-13)
Albert Pérez-Martí, Maite Garcia-Guasch, Anna Tresserra-Rimbau, Alexandra Carrilho-Do-Rosário, Ramon Estruch, Jordi Salas-Salvadó, Miguel Ángel Martínez-González, Rosa Lamuela-Raventós, Pedro F Marrero, Diego Haro, Joana Relat
ABSTRACT

Fibroblast growth factor 21 (FGF21) is considered a promising therapeutic candidate for the treatment of obesity. Since FGF21 production is regulated by various nutritional factors, we analyze the impact of low protein intake on circulating levels of this growth hormone in mice and in a sub cohort of the PREDIMED (Prevención con Dieta Mediterránea) trial. We also describe the role of hepatic FGF21 in metabolic adaptation to a low-protein diet (LPD). We fed control and liver-specific Fgf21 knockout (LFgf21KO) mice a LPD. This diet increased FGF21 production by inducing its overexpression in liver, and this correlated with a body weight decrease without changes in food intake. The LPD also caused FGF21-dependent browning in subcutaneous white adipose tissue (scWAT), as indicated by an increase in the expression of uncoupling protein 1 (UCP1). In a subgroup of 78 individuals from the PREDIMED trial, we observed an inverse correlation between protein intake and circulating FGF21 levels. Our results reinforce the involvement of FGF21 in coordinating energy homeostasis under a range of nutritional conditions. Moreover, here we describe an approach to increase the endogenous production of FGF21, which if demonstrated functional in humans, could generate a treatment for obesity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
0.2 mL
In Stock
Details...
A recombinant, preservative-free antibody is available for your target. Try ZRB1537
$530.00
Sigma-Aldrich
Phosphatase Inhibitor Cocktail 3, DMSO solution
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
0.2 mL
In Stock
Details...
A recombinant, preservative-free antibody is available for your target. Try ZRB1537
$530.00