Skip to Content
MilliporeSigma
  • Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration.

Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration.

Cell death & disease (2021-04-24)
Qianqian Chen, Qianyan Liu, Yunsong Zhang, Shiying Li, Sheng Yi
ABSTRACT

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that stimulates neuronal development and survival. Our previous study has demonstrated that LIF mRNA is dysregulated in the peripheral nerve segments after nerve injury. Here, we show that LIF protein is abundantly expressed in Schwann cells after rat sciatic nerve injury. Functionally, suppressed or elevated LIF increases or decreases the proliferation rate and migration ability of Schwann cells, respectively. Morphological observations demonstrate that in vivo application of siRNA against LIF after peripheral nerve injury promotes Schwann cell migration and proliferation, axon elongation, and myelin formation. Electrophysiological and behavior assessments disclose that knockdown of LIF benefits the function recovery of injured peripheral nerves. Differentially expressed LIF affects the metabolism of Schwann cells and negatively regulates ERFE (Erythroferrone). Collectively, our observations reveal the essential roles for LIF in regulating the proliferation and migration of Schwann cells and the regeneration of injured peripheral nerves, discover ERFE as a downstream effector of LIF, and extend our understanding of the molecular mechanisms underlying peripheral nerve regeneration.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-Thy 1.1 antibody produced in mouse, clone TN-26, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-S-100 (β-Subunit) antibody produced in mouse, clone SH-B1, ascites fluid
Sigma-Aldrich
Anti-Stat3 Antibody, clone 4N11, ZooMAb® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells