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  • Identification of peptidomimetic telomere dysfunction inhibitor (TELODIN) through telomere dysfunction-induced foci (TIF) assay.

Identification of peptidomimetic telomere dysfunction inhibitor (TELODIN) through telomere dysfunction-induced foci (TIF) assay.

STAR protocols (2021-07-02)
Lihui Wang, Zhiguo Wang, Jun-Ping Liu
ABSTRACT

Telomere dysfunction-induced focus (TIF) assay allows efficient profiling of telomere dysfunctions in cells and tissues. Here, we describe the use of the TIF assay to screen synthetic peptides from E3 ubiquitin ligase FBW7, a tumor suppressor gene product, to prevent TIFs caused by environmental radiation stress. We demonstrate peptidomimetic telomere dysfunction inhibitor as a potentially intervening therapeutic drug candidate in aging-related diseases. This work demonstrates a novel utility of the TIF assay protocol in identifying telomere dysfunction inhibitors. For complete details on the use and execution of this protocol, please refer to Wang et al (2020).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Formamide, BioReagent, ≥99.5% (GC), for molecular biology
Sigma-Aldrich
4′,6-Diamidino-2-phenylindole dihydrochloride, powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC), suitable for fluorescence
Sigma-Aldrich
Anti-Goat Serum antibody produced in rabbit, whole antiserum
Roche
Blocking Reagent, For nucleic acid hybridization and detection