Mice Selected for Acute Inflammation Present Altered Immune Response during Pristane-Induced Arthritis Progression.

Mice Selected for Acute Inflammation Present Altered Immune Response during Pristane-Induced Arthritis Progression.

BioMed research international (2018-11-08)

Mara A Correa, Andrea Borrego, José R Jensen, Wafa H K Cabrera, Michele Barros, Iana S S Katz, Tatiane Canhamero, Monica Spadafora-Ferreira, Jussara G Fernandes, Nancy Starobinas, Orlando G Ribeiro, Olga M Ibañez, Marcelo De Franco

PMID30402460

ABSTRACT

Mouse lines selected for maximal (AIRmax) or minimal acute inflammatory reaction (AIRmin) were used to characterize the immune response and the influence of genetic background during pristane-induced arthritis (PIA). Susceptible AIRmax mice demonstrated exacerbated cellular profiles during PIA, with intense infiltration of lymphocytes, as well as monocytes/macrophages and neutrophils, producing higher levels of IL-1β, IFN-γ, TNF-α, IL-10, total IgG3, and chemokines. Resistant AIRmin mice controlled cell activation more efficiently than the AIRmax during arthritis progression. The weight alterations of the spleen and thymus in the course of PIA were observed. Our data suggest that selected AIRmax cellular and genetic immune mechanisms contribute to cartilage damage and arthritis severity, evidencing many targets for therapeutic actions.

MATERIALS

Product Number

Brand

Product Description

MGAMMAG-300K

Millipore

MILLIPLEX^® Mouse Immunoglobulin Isotyping Magnetic Bead Panel - Isotyping Multiplex Assay, Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in murine serum, plasma and cell culture samples.