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Hydrostatic Pressure-Regulated Cellular Calcium Responses.

Langmuir : the ACS journal of surfaces and colloids (2021-01-08)
Minami Fukuchi, Kotaro Oyama, Hiroaki Mizuno, Akihisa Miyagawa, Kazuya Koumoto, Gaku Fukuhara
ABSTRACT

Hydrostatic pressure control has attracted much attention and presents a still challenging objective from mechanobiological viewpoints. Herein, we reveal the calcium entry processes in HeLa cells by means of hydrostatic pressure spectroscopy. The steady-state fluorescence spectral data comprehensively elucidated the factors controlling the outcomes of the hydrostatic pressure-stimulated calcium entry behavior. The present work leads to a new perspective on ion regulations in living cells and an attractive alternative to conventional mechanostimuli.

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Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture