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  • Error-prone protein synthesis recapitulates early symptoms of Alzheimer disease in aging mice.

Error-prone protein synthesis recapitulates early symptoms of Alzheimer disease in aging mice.

Cell reports (2022-09-29)
Margarita Brilkova, Martina Nigri, Harshitha Santhosh Kumar, James Moore, Matilde Mantovani, Claudia Keller, Amandine Grimm, Anne Eckert, Dimitri Shcherbakov, Rashid Akbergenov, Petra Seebeck, Stefanie D Krämer, David P Wolfer, Thomas C Gent, Erik C Böttger
ABSTRACT

Age-related neurodegenerative diseases (NDDs) are associated with the aggregation and propagation of specific pathogenic protein species (e.g., Aβ, α-synuclein). However, whether disruption of synaptic homeostasis results from protein misfolding per se rather than accumulation of a specific rogue protein is an unexplored question. Here, we show that error-prone translation, with its frequent outcome of random protein misfolding, is sufficient to recapitulate many early features of NDDs, including perturbed Ca2+ signaling, neuronal hyperexcitability, and mitochondrial dysfunction. Mice expressing the ribosomal ambiguity mutation Rps9 D95N exhibited disrupted synaptic homeostasis resulting in behavioral changes reminiscent of early Alzheimer disease (AD), such as learning and memory deficits, maladaptive emotional responses, epileptiform discharges, suppressed circadian rhythmicity, and sleep fragmentation, accompanied by hippocampal NPY expression and cerebral glucose hypometabolism. Collectively, our findings suggest that random protein misfolding may contribute to the pathogenesis of age-related NDDs, providing an alternative framework for understanding the initiation of AD.

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Anti-Amyloid Precursor Protein, C-Terminal antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
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