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  • Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH).

Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH).

Methods in molecular biology (Clifton, N.J.) (2022-07-13)
Cedric S Asensio
ABSTRACT

The retention using selective hook (RUSH) system enables us to synchronize and visualize the movement of cargoes along the secretory pathway. A fluorescently tagged cargo of interest is retained in the endoplasmic reticulum and released in a biotin-dependent manner. Here, we report a detailed protocol describing the steps necessary to perform RUSH experiments to study secretory granule biogenesis at the trans-Golgi network (TGN).

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Anti-Insulin antibody, Mouse monoclonal, clone K36AC10, purified from hybridoma cell culture