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A Fungal Protease Model to Interrogate Allergic Lung Immunity.

Methods in molecular biology (Clifton, N.J.) (2018-06-30)
J Morgan Knight, Evan Li, Hui-Ying Tung, Cameron Landers, Jake Wheeler, Farrah Kheradmand, David B Corry
ABSTRACT

Allergic airway diseases (asthma and chronic rhinosinusitis) are among the most common of all human diseases in heavily industrialized societies. Animal models of asthma have provided remarkable insight into allergic disease pathogenesis and will continue to drive the discovery of new therapeutic insights. We provide in this chapter a detailed protocol for inducing allergic immunity in the lungs of mice using a purified fungal protease and include related protocols for assessing immune endpoints.

MATERIALS
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Product Description

Sigma-Aldrich
Protease from Aspergillus oryzae, ≥500 U/g