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  • A new extraction method for Acinetobacter species ODB-L2 rough form lipopolysaccharide from culture broth.

A new extraction method for Acinetobacter species ODB-L2 rough form lipopolysaccharide from culture broth.

Microbios (2001-06-21)
K Kunii, S Nakamura, C Sato, S Fukuoka
ABSTRACT

The Gram-negative bacterium Acinetobacter species ODB-L2 produces lipopolysaccharide (LPS) in culture broth. The LPS could not be purified by conventional extraction methods using 90% phenol/water or 90% phenol/chloroform/petroleum ether mixed solvent. Extraction was achieved employing an admixture of chloroform, ethanol, and 4 M HCI solution. The LPS was purified from dissolving the crude extracts in 90% phenol and LPS sediment formed by addition of methanol. The LPS was characterized by chemical, biochemical, and physicochemical methods as rough form 3-hydroxydodecanoic acid rich LPS.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DL-β-Hydroxylauric acid, ≥99% (GC)