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  • Effect of inclusion body production on culture turbidity and cell dry weight in growing bacterial cultures.

Effect of inclusion body production on culture turbidity and cell dry weight in growing bacterial cultures.

Biotechnology progress (1990-01-01)
S O Hwang, R S Feldberg
ABSTRACT

An approach to the optimization of product yield in an inducible inclusion body-producing system is presented. Following induction by indoleacrylic acid (IAA) of a trpLE-HIVgp41 fusion protein, we found a large increase in culture turbidity and single-cell dry weight. After an initial transition phase, new and constant values for specific growth rate, single-cell light turbidity, and single cell dry weight were achieved, allowing for the determination of optimal conditions for product formation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
trans-3-Indoleacrylic acid, 98%
Sigma-Aldrich
3-Indoleacrylic acid, powder
Sigma-Aldrich
3-Indoleacrylic acid, BioReagent, ≥98% (HPLC)