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  • [Plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of Arthrobacter].

[Plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of Arthrobacter].

Molekuliarnaia genetika, mikrobiologiia i virusologiia (1992-05-01)
S R Agapova, A L Andreeva, I I Starovoĭtov, L I Vorob'eva, P B Terent'ev
ABSTRACT

Arthrobacter crysallopoietes strain KM-4 degrading 2,6-dimethylpyridine and strain KM-4a degrading both 2,6-dimethylpyridine and pyridine, Arthrobacter sp. KM-4b degrading 2,4-dimethylpyridine were isolated from soil. Arthrobacter crystallopoietes KM-4 and Arthrobacter sp. KM-4b contain 100 Md plasmids pBS320 and pBS323. Arthrobacter crystallopietes KM-4a harbours a 100 Md and 80 Md plasmids. Plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine and pyridine. A mutant with lost ability to degrade 2,6-dimethylpyridine was isolated during the growth of strain KM-4 rifR at 42 degrees C. Electrophoretic analysis of the plasmid from temperature sensitive mutant revealed the deletion the size of 26 Md from pBS320 plasmid.

MATERIALS
Product Number
Brand
Product Description

Supelco
2,6-Lutidine, analytical standard
Sigma-Aldrich
2,6-Lutidine
Sigma-Aldrich
2,6-Lutidine, ReagentPlus®, 98%
Sigma-Aldrich
2,4-Lutidine, 99%
Sigma-Aldrich
2,6-Dimethylpyridine, ≥99%