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  • Decalactone production by Yarrowia lipolytica under increased O2 transfer rates.

Decalactone production by Yarrowia lipolytica under increased O2 transfer rates.

Biotechnology letters (2005-10-26)
M Aguedo, N Gomes, E Escamilla Garcia, Y Waché, M Mota, J A Teixeira, I Belo
ABSTRACT

Yarrowia lipolytica converts methyl ricinoleate to gamma-decalactone, a high-value fruity aroma compound. The highest amount of 3-hydroxy-gamma-decalactone produced by the yeast (263 mg l(-1)) occurred by increasing the k(L)a up to 120 h(-1) at atmospheric pressure; above it, its concentration decreased, suggesting a predominance of the activity of 3-hydroxyacyl-CoA dehydrogenase. Cultures were grown under high-pressure, i.e., under increased O(2) solubility, but, although growth was accelerated, gamma-decalactone production decreased. However, by applying 0.5 MPa during growth and biotransformation gave increased concentrations of dec-2-en-4-olide and dec-3-en-4-olide (70 mg l(-1)).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
γ-Decalactone, ≥98%, FCC, FG
Supelco
Methyl ricinoleate, analytical standard
Sigma-Aldrich
γ-Decanolactone, 98%
Sigma-Aldrich
Methyl ricinoleate, ≥99% (GC)