- Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae.
Properties of a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae.
Bioscience, biotechnology, and biochemistry (2006-07-25)
Takahiro Oba, Yoshitsugu Yamamoto, Shuji Nomiyama, Hikaru Suenaga, Shigeru Muta, Kosuke Tashiro, Satoru Kuhara
PMID16861814
ABSTRACT
We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.
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