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  • Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry.

Measurement of aflatoxin and aflatoxin metabolites in urine by liquid chromatography-tandem mass spectrometry.

Journal of analytical toxicology (2007-06-21)
Robert A Everley, Frederic L Ciner, Dongliang Zhan, Peter F Scholl, John D Groopman, Timothy R Croley
ABSTRACT

Automated immunoaffinity solid-phase extraction followed by liquid chromatography-tandem mass spectrometry and chemical analogue internal standardization is employed to detect and quantify the aflatoxins AFB(1), AFB(2), AFG(1), AFG(2), and the metabolites AFM(1) and AFP(1) in urine. The dynamic range of the method is nearly three orders of magnitude with limits of detection in the low femtogram on column range. The method was validated over a 12-day period by eight analysts. This method is suitable for agricultural, forensic, and public health laboratories during an accidental outbreak or a chemical terrorism event where a rapid and accurate diagnosis of aflatoxicosis is needed.

MATERIALS
Product Number
Brand
Product Description

Supelco
Aflatoxin G2 solution, 0.5 μg/mL in acetonitrile, analytical standard
Aflatoxin G2 solution, 3.80 μg/g in acetonitrile, ERM®, certified reference material
Sigma-Aldrich
Aflatoxin G2