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  • [Optimization of determination of aflatoxins in foods with bromine postcolumn derivatization].

[Optimization of determination of aflatoxins in foods with bromine postcolumn derivatization].

Roczniki Panstwowego Zakladu Higieny (2008-02-06)
Ludwik Czerwiecki, Grazyna Wilczyńska
ABSTRACT

The method for determination of aflatoxin B1, B2, G1 i G2 in nuts, culinary spices, cereals and cereal products was described. To optimize the analytical procedure in several products, condition of proper extraction, clean-up, HPLC and detection were selected. After extraction by means of methanol and water (80+20 v/v) or (70+30 v/v), clean-up on IAC columns, HPLC on C18 columns--Nucleosil and Nova Pak with mobile phase-methanol, acetonitrile, water (20+20+60 v/v) was performed. For fluorometric detection at 362/430 nm, post-column derivatization of aflatoxin B1 and G1 with bromine was carried out. The mean recovery of the method depending on matrix and aflatoxin, was 52-102% at RSD% 0.2-8.3. LOD and LOQ, respectively were: 0.01 and 0.02 microg/kg for nuts and 0.05 and 0.1 microg/kg for culinary spices and cereal products. The concentrations of aflatoxins in 86 samples of foods from market were below the permissible maximum levels legally binding.

MATERIALS
Product Number
Brand
Product Description

Supelco
Aflatoxin G2 solution, 0.5 μg/mL in acetonitrile, analytical standard
Aflatoxin G2 solution, 3.80 μg/g in acetonitrile, ERM®, certified reference material
Sigma-Aldrich
Aflatoxin G2