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Prostaglandin E1 transport into rat intestinal cryptic cell line IEC-6.

Research communications in molecular pathology and pharmacology (2008-04-23)
Kohji Hirata, Kumiko Mabashi, Toshiharu Horie
ABSTRACT

Administration of antitumor drugs such as 5-fluorouracil and methotrexate to rats causes intestinal injury. The intestinal injury was reported to be prevented by coadministration of prostaglandin E1 analogues to rats. IEC-6 cell is a useful in vitro model to study the cytoprotective effect in enterocytes, For its purpose, it is important to know whether or not prostaglandins are taken up into IEC-6 cells. The transport of prostaglandin E1 into IEC-6 cells was investigated, using [3H]-prostaglandin E1. Prostaglandin E1 was found to be transported into IEC-6 cells by the passive diffusion mechanism.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bromocresol Green, ACS reagent, Dye content 95 %
Sigma-Aldrich
Bromocresol Green Sultone Form, for microscopy (Bot., Hist., Vit.), indicator (pH 3.8-5.4)