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  • Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester.

Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester.

Journal of bioscience and bioengineering (2009-08-12)
Ikuo Kira, Yasuhisa Asano, Kenzo Yokozeki
ABSTRACT

Screening was carried out for microorganisms able to produce N-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing APM production was purified and identified as dipeptidase E.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-Phenylalanine methyl ester hydrochloride, 98%
Sigma-Aldrich
L-Phenylalanine methyl ester hydrochloride, 98%