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  • Protein resistance of PNIPAAm brushes: application to switchable protein adsorption.

Protein resistance of PNIPAAm brushes: application to switchable protein adsorption.

Langmuir : the ACS journal of surfaces and colloids (2009-09-22)
Sina Burkert, Eva Bittrich, Marco Kuntzsch, Martin Müller, Klaus-Jochen Eichhorn, Cornelia Bellmann, Petra Uhlmann, Manfred Stamm
ABSTRACT

Protein adsorption, as the primary process occurring when a foreign surface comes into contact with a biosystem, was studied on thin polymer brush films consisting of poly(N-isopropylacrylamide) (PNIPAAm) and poly(2-vinylpyridine) (P2VP). These films were prepared by the "grafting to" method. The protein resistance of stimuli responsive PNIPAAm-brushes toward serum albumin was recorded and compared with protein adsorption on P2VP brushes. To achieve a better understanding of protein resistance, PNIPAAm brushes with different molecular weights were investigated below and above the lower critical solution temperature of 32 degrees C. To use these findings for the adjustment and switching of protein adsorption, in a first attempt the adsorption on a mixed brush system consisting of PNIPAAm and P2VP chains was studied. This system showed temperature-dependent adsorption behavior due to the presence of PNIPAAm, representing a smart surface with stimuli-responsive changes in the physicochemical surface properties. With this mixed brush, the adsorbed amount of protein could be controlled, depending on composition and the temperature of the surroundings.

MATERIALS
Product Number
Brand
Product Description

Supelco
Poly(2-vinylpyridine), analytical standard, average Mw 159,000 (Typical), average Mn 152,000 (Typical)
Supelco
Poly(2-vinylpyridine), analytical standard, average Mw 37,500 (Typical), average Mn 35,000 (Typical)