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  • Nitrophenylboronic acids as highly chemoselective probes to detect hydrogen peroxide in foods and agricultural products.

Nitrophenylboronic acids as highly chemoselective probes to detect hydrogen peroxide in foods and agricultural products.

Journal of agricultural and food chemistry (2011-10-05)
Chun-Ping Lu, Chieh-Ti Lin, Ching-Ming Chang, Shih-Hsiung Wu, Lee-Chiang Lo
ABSTRACT

Hydrogen peroxide is commonly used in the food processing industry as a chlorine-free bleaching and sterilizing agent, but excessive amounts of residual hydrogen peroxide have led to cases of food poisoning. Here we describe the development of a novel nonenzymatic colorimetric method for the determination of residual hydrogen peroxide in foods and agricultural products. Nitrophenylboronic acids chemoselectively react with hydrogen peroxide under alkaline conditions to produce yellow nitrophenolates. Of the three nitrophenylboronic acid isomers tested, the p-isomer displayed the highest sensitivity for hydrogen peroxide and the fastest reaction kinetics. The reaction product, p-nitrophenolate, has an absorption maximum at 405 nm and a good linear correlation between the hydrogen peroxide concentration and the A(405) values was obtained. We successfully applied this convenient and rapid method for hydrogen peroxide determination to samples of dried bean curds and disposable chopsticks, thereby demonstrating its potential in foods and agricultural industries.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
3-Nitrophenylboronic acid, ≥97%