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  • Selective and rapid solubilization of the microbial membrane enzyme aldehyde dehydrogenase.

Selective and rapid solubilization of the microbial membrane enzyme aldehyde dehydrogenase.

Journal of basic microbiology (1990-01-01)
R Hommel, H P Kleber
ABSTRACT

An improved solubilization procedure for the membrane-bound quinoprotein aldehyde dehydrogenase from Acetobacter rancens CCM 1774 was established. After the first solubilization of membrane enzymes by Brij 35 which provided important extraction of membrane proteins other than aldehyde dehydrogenase, the application of Trition X-100 resulted in an almost 20-fold purification of quinoprotein aldehyde dehydrogenase. The optimal solubilization was closely connected with definite detergent/protein ratios.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
ECO Brij® L23
Sigma-Aldrich
Brij® L23, main component: tricosaethylene glycol dodecyl ether