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Transformation of malathion by Lysinibacillus sp. isolated from soil.

Biotechnology letters (2012-04-06)
Baljinder Singh, Jagdeep Kaur, Kashmir Singh
ABSTRACT

An axenic bacterial strain, Lysinibacillus sp. KB1, was isolated from malathion-contaminated soil. It tolerated malathion up to 0.15 % and, under aerobic conditions, utilized it as sole carbon source. 20 % malathion and 47 % malaoxon were degraded out of the initially provided malathion. Two metabolites, mal-monocarboxylic acid and mal-dicarboxylic acid, were detected within 7 days at 30 °C. Esterase activity of the strain was 240 ± 2.5 U/ml after 7 days of growth. Sterilized soil mixed with malathion showed rapid degradation of malathion when inoculated with strain KB1 as compared to the uninoculated soil.

MATERIALS
Product Number
Brand
Product Description

Supelco
Malaoxon, PESTANAL®, analytical standard
Malathion impurity B, European Pharmacopoeia (EP) Reference Standard