MilliporeSigma
  • Proteoglycans from Boswellia serrata Roxb. and B. carteri Birdw. and identification of a proteolytic plant basic secretory protein.

Proteoglycans from Boswellia serrata Roxb. and B. carteri Birdw. and identification of a proteolytic plant basic secretory protein.

Glycobiology (2012-07-10)
Andreas Herrmann, Simone König, Matthias Lechtenberg, Maria Sehlbach, Sergey Y Vakhrushev, Jasna Peter-Katalinic, Andreas Hensel
ABSTRACT

Water-soluble high molecular weight compounds were isolated in yields of 21-22% from the oleogum of Boswellia serrata and B. carteri. Using anion exchange chromatography and gel permeation chromatography, different proteoglycans were purified and characterized, leading to four principally different groups: (i) Hyp-/Ser-rich extensins with O-glycosidic attached arabinan side chains; (ii) Modified extensins, with arabinogalactosylated side chains containing GlA and 4-O-Me-GlcA; (iii) Glycoproteins with N-glycosidic side chains containing higher amounts of Fuc, Man and GluNH(2,) featuring a 200 kD metalloproteinase that has been de novo sequenced and is described for the first time; (iv) Type II arabinogalactans-proteins. Significant differences between the gums from the two species were observed in the protein content (6% vs 22%), offering the possibility of a quick differentiation of gums from both species for analytical quality control. The data also offer an insight into the plant response towards wound-closing by the formation of extensin and AGP-containing gum.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(+)-Arabinogalactan, ≥80% (HPLC), from larch wood
Supelco
(+)-Arabinogalactan, for use with Total Dietary Fiber Control Kit, TDF-C10, from larch wood