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  • Extracellular neutral lipids produced by the marine bacteria Marinobacter sp.

Extracellular neutral lipids produced by the marine bacteria Marinobacter sp.

Biocontrol science (2012-07-14)
Miyo Nakano, Shunpei Iehata, Reiji Tanaka, Hiroto Maeda
ABSTRACT

We analyzed the production of neutral lipids by the marine hydrocarbonoclastic bacteria Marinobacter sp. strain PAD-2 using hexadecane or succinate as the sole carbon source. Results showed that strain PAD-2 was able to grow and reduce the surface tension to 33±1.5 mN m(-1) and 58±1.5 mN m(-1) when n-hexadecane or succinate was used as the sole carbon source, respectively. The lipophilic compounds produced by Marinobacter sp. strain PAD-2 were extracted, and then crude lipophilic compounds, expected to be wax ester-like lipids, were analyzed by thin layer chromatography (TLC) . Furthermore, the lipophilic compound demonstrating surface activity was purified and subjected to gas chromatography/mass spectrometry (GC/MS) analysis. Although these did not give definite structural information due to the weak molecular ion peak (M(+)) , one component Ma-1 showed almost the same mass spectrum as that of component Fa-2, which represented a biosurfactant derived from Dietzia maris reported previously. Cell hydrophobicity was measured by a test of bacterial adhesion to hydrocarbons. A higher hydrophobic cell surface was observed in strain PAD-2. Extracellular wax ester-like compounds seem to be one type of the surface active compounds when bacteria grow on hexadecane or succinate as the sole carbon source.

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Sigma-Aldrich
Hexadecane, ReagentPlus®, 99%
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500 g
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Supelco
Hexadecane, analytical standard
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Sigma-Aldrich
Hexadecane, anhydrous, ≥99%
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SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$77.70
1 kg
In Stock
Details...
$136.00