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  • Capillary electrophoretic separations of proteins using nonionic surfactant coatings.

Capillary electrophoretic separations of proteins using nonionic surfactant coatings.

Analytical chemistry (1991-06-01)
J K Towns, F E Regnier
ABSTRACT

Capillary zone electrophoretic separations of proteins have been achieved by using nonionic surfactant coated capillaries. Capillaries were prepared by derivatization of the silica surface with octadecylsilane followed by the deposition of a layer of nonionic surfactant from an aqueous solution above the critical micelle concentration. This coating is of sufficient thickness and hydrophilicity to reduce both protein adsorption and electroosmotic pumping. This hydrophilic coating reduces electroosmotic pumping 5-8-fold while resolving proteins quickly and efficiently with good recovery. The coating provides a stable and reproducible means of deactivation, while the rate of electroosmotic pumping stays relatively constant throughout the pH range 4-11. This allows the pH to be varied to enhance selectivity without adversely affecting the flow rate.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
ECO Brij® L23
Sigma-Aldrich
Brij® L23, main component: tricosaethylene glycol dodecyl ether