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Functionalizing bacterial cell surfaces with a phage protein.

Chemical communications (Cambridge, England) (2012-12-19)
Yi-Chun Yeh, Jana Müller, Changhao Bi, Nathan J Hillson, Harry R Beller, Swapnil R Chhabra, Steven W Singer
ABSTRACT

Functionalization of bacterial cell surfaces has the potential to introduce new activities by chemical modification. Here we show that a bacteriophage-receptor complex can be used to functionalize the surface of two Gram-negative proteobacteria, Escherichia coli and Ralstonia eutropha with CdSe/ZnS nanoparticles. This work highlights the potential for using microbe-phage interactions to generate new functions on living cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Zinc sulfide, purum, 97% (from Zn)
Sigma-Aldrich
Zinc sulfide, pieces, 3-12 mm, 99.9% trace metals basis
Sigma-Aldrich
Zinc sulfide, powder, 10 μm, 99.99% trace metals basis