MilliporeSigma
  • Preparation and characterization of a photocrosslinkable bioadhesive inspired by marine mussel.

Preparation and characterization of a photocrosslinkable bioadhesive inspired by marine mussel.

Journal of photochemistry and photobiology. B, Biology (2013-01-15)
Jie Xue, Tao Wang, Jun Nie, Dongzhi Yang
ABSTRACT

Synthetic adhesives inspired by marine mussel have attracted great attention due to its excellent water-resistance and good biocompatibility. In this study, a photocrosslinkable bioadhesive containing 3,4-Dihydroxy-l-phenylalanine (DOPA) functional group, which is central to curing mussel adhesive proteins, was prepared by ultraviolet (UV) irradiation of a new photocurable monomer ethylene glycol acrylate methacrylate-dopamine (EGAMA-DOPA) and a UV photocrosslinkable crosslinking agent poly(vinyl alcohol) (UV-PVA) derivative. The chemical structures of EGAMA-DOPA and UV-PVA were confirmed by Fourier Transform Infrared Reflection (FTIR) and (1)H NMR spectroscopy, respectively. The effects of UV light intensity, content of photoinitiator, EGAMA-DOPA and UV-PVA on the photopolymerization kinetics were studied, and the effects of the content of UV-PVA and temperature on the adhesive strength were also investigated. It was found that the higher UV light intensity, the faster polymerization rate and the higher final conversion that was the same as the trend of photoinitiator, EGAMA-DOPA and UV-PVA. And the adhesion strength measurement showed that, for gels with 30wt.% EGAMA-DOPA, the adhesion strength was obviously improved by about 150% with 3.0wt.% UV-PVA instead of pure PVA, and for gels containing 40wt.% EGAMA-DOPA, the adhesion strength sharply enhanced by 123% with increasing the content of UV-PVA from 1.0wt.% to 3.0wt.%. Cell attachment results showed good cell viability of L929 cell on the EGAMA-DOPA/UV-PVA adhesive gels. Thanks to its strong adhesion strength and good biocompatibility, such photocrosslinkable gels could be applied to the areas of biomedical field.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mowiol® 6-98, Mw ~47,000
Sigma-Aldrich
Mowiol® 10-98, Mw ~61,000
Sigma-Aldrich
Poly(vinyl alcohol), Fully hydrolyzed
Sigma-Aldrich
3,4-Dihydroxy-DL-phenylalanine
Sigma-Aldrich
Poly(vinyl alcohol), 87-90% hydrolyzed, average mol wt 30,000-70,000
Sigma-Aldrich
Mowiol® 8-88, Mw ~67,000
Sigma-Aldrich
Mowiol® 4-88, Mw ~31,000
Sigma-Aldrich
Poly(vinyl alcohol), Mw 146,000-186,000, 99+% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), average Mw 85,000-124,000, 87-89% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), average Mw 146,000-186,000, 87-89% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), Mw 85,000-124,000, 99+% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), average Mw 31,000-50,000, 87-89% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), Mw 31,000-50,000, 98-99% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), Mw 13,000-23,000, 87-89% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), Mw 89,000-98,000, 99+% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), average Mw 13,000-23,000, 98% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), Mw 9,000-10,000, 80% hydrolyzed
Sigma-Aldrich
Poly(vinyl alcohol), average Mw 130,000, 99+% hydrolyzed
Sigma-Aldrich
Mowiol® 4-98, Mw ~27,000
Sigma-Aldrich
Mowiol® 20-98, Mw ~125,000
Sigma-Aldrich
Mowiol® 40-88, average Mw ~205,000 g/mol
Sigma-Aldrich
Mowiol® 18-88, Mw ~130,000
Sigma-Aldrich
Mowiol® 56-98, Mw ~195,000
Sigma-Aldrich
Mowiol® 28-99, Mw ~145,000