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  • Degradation of ascorbic acid and potassium sorbate by different Lactobacillus species isolated from packed green olives.

Degradation of ascorbic acid and potassium sorbate by different Lactobacillus species isolated from packed green olives.

Food microbiology (2013-03-19)
Alfredo Montaño, Antonio Higinio Sánchez, Francisco Javier Casado, Víctor Manuel Beato, Antonio de Castro
ABSTRACT

The aim of this research was to ascertain the lactic acid bacteria responsible for the degradation of ascorbic acid and/or potassium sorbate, isolated from packed green olives where these additives had diminished. A total of 14 isolates were recovered from samples of different green olive containers. According to partial sequencing of the 16S rRNA coding gene, Lactobacillus parafarraginis, Lactobacillus rapi, Lactobacillus pentosus, Lactobacillus paracollinoides, and Pediococcus ethanolidurans were identified. With the exception of L. pentosus and L. paracollinoides, the other species had not been mentioned in table olives before this study. Only three of the 14 isolates metabolized ascorbic acid in MRS broth, and the products from ascorbic acid in modified MRS broth without carbon sources were acetic and lactic acids. Except for the two L. rapi and the two P. ethanolidurans strains, the remaining 10 isolates depleted potassium sorbate added into MRS broth to some extent. The product generated by three of these strains was confirmed to be trans-4-hexenoic acid. The degradation of ascorbate or sorbate by lactic acid bacteria should be taken into account when these additives are used in food products where this group of bacteria may be present.

MATERIALS
Product Number
Brand
Product Description

Sorbic acid, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Sorbic acid, tested according to Ph. Eur.
Sigma-Aldrich
Sorbic acid, 99.0-101.0% anhydrous basis