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  • Inhibition kinetics of chloramphenicol acetyltransferase by selected detergents.

Inhibition kinetics of chloramphenicol acetyltransferase by selected detergents.

Biochemical and biophysical research communications (1993-10-15)
J Lu, C Jiang
ABSTRACT

Kinetic analyses indicate that the inhibitory effects of the nonionic detergents Triton X-100 and Nonidet P-40 on chloramphenicol acetyltransferase are exerted by a competitive and a non-competitive mechanism with respect to the substrates chloramphenicol and acetyl-CoA, respectively. Comparison with nonionic detergents without an aromatic moiety like that present in Triton X-100 and Nonidet P-40 suggests that the aromatic groups in these two detergents may compete with chloramphenicol for binding to the hydrophobic, active site in the chloramphenicol acetyltransferase.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
n-Dodecyl β-D-glucopyranoside, ≥98% (GC)