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  • Expression of beta-galactosidase from a hybrid promoter:operator element in Escherichia coli.

Expression of beta-galactosidase from a hybrid promoter:operator element in Escherichia coli.

FEMS microbiology letters (1993-01-15)
K E Dixon, D F Fix
ABSTRACT

A hybrid trpPO:lacO regulatory sequence was cloned upstream of a promoterless lacZ gene and recombined onto a lambda bacteriophage. Escherichia coli lysogens representing the four possible phenotypes for lacI and trpR were constructed and the synthesis of beta-galactosidase was assayed under various growth conditions. The results illustrated that both control elements could be efficiently and independently regulated by the addition or omission of appropriate accessory molecules.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
trans-3-Indoleacrylic acid, 98%
Sigma-Aldrich
3-Indoleacrylic acid, BioReagent, ≥98% (HPLC)
Sigma-Aldrich
3-Indoleacrylic acid, powder