MilliporeSigma

Synthesis of branched polysaccharides with tunable degree of branching.

Carbohydrate polymers (2013-03-08)
Jelena Ciric, Katja Loos
ABSTRACT

An in vitro enzyme-catalyzed tandem reaction using the enzymes phosphorylase b from rabbit muscle and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) to obtain branched polyglucans with tunable degree of branching (2% ÷ 13%) is presented. The tunable degree of branching is obtained by varying the reaction conditions such as pH value, the choice of reducing agent and its concentration and reaction time. Linear amylose is formed by the phosphorylase-catalyzed propagation of glucose-1-phosphate while Dg GBE introduces branching points on the α-(1→6) position by relocating short oligosaccharide chains. Our results show that the best way to obtain different degrees of branching with this set of enzymes is by regulation of the reaction time.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
α-D-Glucose 1-phosphate dipotassium salt hydrate, ≥97% (HPLC)
Sigma-Aldrich
α-D-Glucose 1-phosphate disodium salt hydrate, ≥97% (Enzymatic Purity, anhydrous)
Sigma-Aldrich
α-D-Glucose 1-phosphate dipotassium salt hydrate, ≥99% (HPLC), BioXtra