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  • Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica.

Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica.

Biochemical and biophysical research communications (2013-06-04)
Kentaro Kato, Yasuhiro Takegawa, Katherine S Ralston, Carol A Gilchrist, Shinjiro Hamano, William A Petri, Yasuro Shinohara
ABSTRACT

Mouse strain-specific differences in the carbohydrate composition of intestinal mucins were hypothesized to account for strain-dependent susceptibility to Entamoeba histolytica. To test this hypothesis, intestinal mucins from susceptible and resistant inbred strains of mice were analyzed for their O-glycan content and for their ability to inhibit amoebic adherence to (GalNAc)12-27-HSA neo-glycoproteins. The results showed that the colorectal mucin O-glycan of susceptible CBA mice was lower in sialic acid content than that of resistant C57BL/6 and BALB/c mice. Mucins from CBA mice were more potent inhibitors of E. histolytica adherence to neo-glycoproteins than were mucins from C57BL/6 or BALB/c mice. Consistent with the role of terminal Gal/GalNAc as a receptor for amoebic adherence, sialidase treatment of C57BL/6 and BALB/c colorectal mucins increased their ability to inhibit E. histolytica adherence to the neo-glycoproteins. These results provide evidence of mouse strain-specific differences in the sialic acids content of mucin O-glycans. These dissimilarities likely contribute to the differential susceptibility of the three mouse strains to E. histolytica infection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N-Acetylneuraminic acid, ≥95% anhydrous basis, synthetic
Sigma-Aldrich
N-Acetylneuraminic acid, ≥98% (HPLC), from Escherichia coli
Sigma-Aldrich
Protease from Aspergillus oryzae, ≥500 U/g