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  • OT-II TCR transgenic mice fail to produce anti-ovalbumin antibodies upon vaccination.

OT-II TCR transgenic mice fail to produce anti-ovalbumin antibodies upon vaccination.

Cellular immunology (2013-06-19)
Shelly Leung, Douglas Smith, Andrzej Myc, Jingga Morry, James R Baker
ABSTRACT

OT-II mice were evaluated as a transgenic strain-specific model to assess T-cell help for B-cell responses. OT-II CD4(+) T-cells express transgenic OVA-specific αβ-TCRs. This high frequency of antigen-specific helper T-lymphocytes may augment induction of B-cell responses. Unexpectedly, OT-II mice did not produce OVA-specific antibodies after intranasal immunization. However, B-cells expressed normal antigen-presenting function in vitro for activation of OVA-specific T-cell responses. These OT-II T-cell responses produced a Th1-type cytokine profile with significantly reduced Th2 or Th17 responses. These data suggest that OT-II B-cells are not defective as APCs, however, downstream antibody responses are abrogated in this transgenic strain.

MATERIALS
Product Number
Brand
Product Description

Supelco
Albumin from chicken egg white, For use as a marker in SDS-PAGE
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder
Sigma-Aldrich
Albumin from chicken egg white, powder, 62-88% (agarose gel electrophoresis)
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder, ≥90% (agarose gel electrophoresis)
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)