• Di-n-butyl phthalate causes antiestrogenic effects in female Murray rainbowfish (Melanotaenia fluviatilis).

Di-n-butyl phthalate causes antiestrogenic effects in female Murray rainbowfish (Melanotaenia fluviatilis).

Environmental toxicology and chemistry (2013-06-14)
Harpreet Bhatia, Anupama Kumar, Jun Du, John Chapman, Mike J McLaughlin

Di-n-butyl phthalate (DnBP) is an industrial pollutant with antiandrogenic effects reported in male mammals and fish. Little research has been done on the endocrine effects of DnBP in female fish. The present study investigated the changes in ovarian histology and serum vitellogenin concentrations in adult Murray rainbowfish (Melanotaenia fluviatilis) after exposure to 125 µg/L, 250 µg/L, 500 µg/L, and 1000 µg/L DnBP for 7 d. Treatment at 125 µg/L to 1000 µg/L DnBP for 7 d had no significant effect on the survival, condition factor, gonadosomatic index, hepatosomatic index, and developmental stage of the fish. Based on the histological investigation, the sizes of the previtellogenic oocytes in the fish treated at 250 µg/L to 1000 µg/L were found to be significantly higher than in the corresponding control fish (p ≤ 0.05). The early vitellogenic oocytes in the fish treated at 1000 µg/L were significantly smaller relative to those in the unexposed fish (p ≤ 0.05). Histological changes like chorion folding, shrunken ooplasm, impaired yolk production, granulomatous inflammation, and interstitial fibrosis were observed in the ovaries of the fish treated with DnBP. The circulating levels of plasma vitellogenin were significantly lower in the fish exposed to 500 µg/L and 1000 µg/L DnBP (p ≤ 0.05). These data show that a continuous exposure to subacute concentrations of DnBP for 7 d can cause antiestrogenicity in female adult Murray rainbowfish.

Product Number
Product Description

Dibutyl phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Dibutyl phthalate, 99%
Di-n-butyl phthalate solution, certified reference material, 5000 μg/mL in methanol
Dibutyl phthalate, PESTANAL®, analytical standard