Skip to Content
MilliporeSigma
  • Therapeutic effects of anti-B7-H3 antibody in an ovalbumin-induced mouse asthma model.

Therapeutic effects of anti-B7-H3 antibody in an ovalbumin-induced mouse asthma model.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology (2013-09-24)
Zheng-Rong Chen, Guang-Bo Zhang, Yu-Qing Wang, Yong-Dong Yan, Wei-Fang Zhou, Canhong Zhu, Ying Chen, Jian Wang, Wei Ji
ABSTRACT

B7 molecules play a key role in regulating allergen-induced T cell activation in asthma, which may occur through T cell recruitment and T helper cell differentiation on allergen provocation. Initial studies have shown that B7-H3 (CD276), a recently identified B7 family member, plays a critical role in the development of Th2 cells. To investigate the effects of anti-B7-H3 monoclonal antibody (mAb) in a mouse model of allergic asthma. The asthma model was established by ovalbumin (OVA) sensitization and challenging in female BALB/c mice. Total cell numbers in bronchoalveolar lavage fluid (BALF) were determined, and the expression levels of interferon gamma (IFN-γ), interleukin (IL)-4, and IL-17 in BALF were measured by enzyme-linked immunosorbent assay. Pulmonary eosinophil infiltration and mucus production were detected by hematoxylin and eosin (H&E) and periodic acid-Schiff (PAS), respectively. B7-H3 expression was detected by immunohistochemistry in frozen tissue sections. Anti-B7-H3 mAb treatment alleviated the asthmatic syndrome, decreased the levels of B7-H3-positive cells in the lung tissues, abrogated hypercellularity, eosinophil infiltration, and mucus production, and inhibited IL-4 and IL-17 production in BALF at the induction phase as compared with the immunoglobulin G (IgG) control group (P < .01). In addition, the treatment of anti-B7-H3 mAb at the induction phase could increase the expression levels of IFN-γ as compared with the IgG control group (P < .01). Anti-B7-H3 mAb treatment at the effector phase did not inhibit the asthma response. Blockade of B7-H3 signals may provide a novel therapeutic approach to the treatment of allergic asthma.

MATERIALS
Product Number
Brand
Product Description

Supelco
Albumin from chicken egg white, For use as a marker in SDS-PAGE
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder
Sigma-Aldrich
Albumin from chicken egg white, powder, 62-88% (agarose gel electrophoresis)
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder, ≥90% (agarose gel electrophoresis)
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)