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  • Involvement of a chromatin modifier in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell injury: probably an indirect action via the regulation of NFκB/FasL circuitry.

Involvement of a chromatin modifier in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell injury: probably an indirect action via the regulation of NFκB/FasL circuitry.

Biochemical and biophysical research communications (2013-10-15)
Shiwei Chen, Yushu Dong, Chun Xu, Liming Jiang, Yongjie Chen, Cheng Jiang, Wugang Hou, Wei Li
ABSTRACT

The Fas/FasL signaling pathway, controlled by nuclear factor-κB (NFκB) at the transcriptional level, is critical for triggering germ cell apoptosis in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell (SC) injury, but the exact regulation mechanism remain unknown. Here, we discovered that expression level of Metastasis associated protein 1 (MTA1), a component of the Mi-2/nucleosome remodeling and deacetylase complex, was upregulated in SCs during the early recovery after MEHP exposure. This expression change was in line with the dynamic changes in germ cell apoptosis in response to MEHP treatment. Furthermore, a knockdown of MTA1 by RNAi in SCs was found to impair the MEHP-induced early activation of NFκB pathway and abolish the recruitment of NFκB onto FasL promoter, which consequently diminished the MEHP-triggered FasL induction. Considering that Fas/FasL is a well characterized apoptosis initiating signaling during SCs injury, our results point to a potential "switch on" effect of MTA1, which may govern the activation of NFκB/FasL cascade in MEHP-insulted SCs. Overall, the MTA1/NFκB/FasL circuit may serve as an important defensive/repairing mechanism to help to control the germ cell quality after SCs injury.

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Dioctyl phthalate, ≥99.5%
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100 μg
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Bis(2-ethylhexyl) phthalate, Selectophore
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100 μg
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Bis(2-ethylhexyl) phthalate, PESTANAL®, analytical standard
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100 μg
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Bis(2-ethylhexyl) phthalate, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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100 μg
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Bis(2-ethylhexyl) phthalate, SAJ first grade, ≥98.0%
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100 μg
Estimated to ship on March 22, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB1515
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Supelco
Bis(2-ethylhexyl) phthalate solution, certified reference material, 2000 μg/mL in methanol
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100 μg
Estimated to ship on March 22, 2025
A recombinant, preservative-free antibody is available for your target. Try ZRB1515
$484.00